GENETIC PROFILING AND DIVERSITY OF SOME PROMISING EFFICIENT RHIZOBIAL ISOLATES ON FABA BEAN PLANTS

A group of twenty faba bean rhizobial isolates was collected from two Egyptian Governorates (Dakahlia and Damitta). The were further morphologically physiologically characterized to check their growth symbiotic performance on plants. According remarkable lab pots tests, five (Rh 32, Rh 6-A, 3-4, RL3, 8-A) selected subjected biochemical molecular characterizations. Genetic profiling the promising conducted using six ISSR-primers. Amplification bacterial genomic DNA produced a total 37 loci, 54% them polymorphic 46% monomorphic. rate polymorphism ranged between 25% 80% with an average 54%. Clustering pattern analysis morphological physiological data grouped in clusters falling close each other. ISSR four clusters. Analysis based revealed that lowest genetic distance 2.00 6-A 3-4 isolates, while highest 3.61 32 RL3 isolates. greatest similarity measurement 0.931 isolates; 0.745 It can concluded clustering could be used facilitating selection will as source genes for biological nitrogen fixation plant growth-promotion